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Author: KBI Admin

Meet Our Members – Tofino Kombucha (Canada)

  Kelsey Hendricks, Tofino Kombucha, Tofino, BC, Canada Q: When did you first discover kombucha? In 2013, while making a deposit at a bank, I asked the teller (who was a friend of mine) what she was drinking that day. The kombucha bottle was empty, but she let me smell the strong vinegary fumes still […]


Monday, 16th July 2018

 

Kelsey Hendricks, Tofino Kombucha, Tofino, BC, Canada

Q: When did you first discover kombucha?

In 2013, while making a deposit at a bank, I asked the teller (who was a friend of mine) what she was drinking that day. The kombucha bottle was empty, but she let me smell the strong vinegary fumes still emitting from the bottle. Wow! I believed her when she said it was a health drink and that I should go to the store and buy a bottle. I tried it and I loved it! Soon, I had tried every brand and flavour sold in my small town, but I wanted more. Now it was time to try making it myself.

Q: Why did you start your own kombucha company?

Kombucha brewing really is an art form and when you love creating something you end up wanting to share your creation. Starting a kombucha company was a small idea that began to snowball and before you know my research into whether I should start a kombucha business turned into me ACTUALLY starting a kombucha business. It was a long process to get the business from an idea to a real thing living and growing in my basement. As exciting as it is to put a label on my bottle and see it leave for the grocery store and then see someone drinking and enjoying it, it’s even more exciting knowing that I am sharing something healthy that I love to make and hopefully it will help inspire more people to make healthier eating choices, including more fermented foods.

Q: What is your favorite flavor of kombucha? 

Ferrytale Rose by Raincoast Kombucha from Powell River, BC (made with dried cherries, green rooibos and rose petals).

Q: What is your former/current other life or career?

Former career: I have a background in accounting, administration, business, hospitality, and food service – all of which have been very useful in starting a beverage company. Other life: I am a mom, a wife, a gardener, a sourdough bread maker, an okay cook, a runner, a surfer, a hiker, a camper and lover of the outdoors.

Q: What is a saying that you live by?

Always be open to learning because sometimes you don’t know what you don’t know.

 

 

 

 

 

Meet Our Members – Circle City Kombucha (IN)

  Skylar Williams, Circle City Kombucha, Indianapolis, IN, USA Q: When did you first discover kombucha? My mother turned me onto Kombucha in 2006, after she was diagnosed with breast cancer. She had read about GT Dave’s mother and her usage of Kombucha while battling the disease. Q: Why did you start your own kombucha […]


Monday, 16th July 2018

 

Skylar Williams, Circle City Kombucha, Indianapolis, IN, USA

Q: When did you first discover kombucha?

My mother turned me onto Kombucha in 2006, after she was diagnosed with breast cancer. She had read about GT Dave’s mother and her usage of Kombucha while battling the disease.

Q: Why did you start your own kombucha company?

Years ago I was trying out various forms of creativity, and Kombucha turned out to be something I enjoyed making. Eventually friends, including one of our co-founders, noticed I was making some good booch, and asked if I ever thought I might do something with it. The answer was, “YES,” and the rest is history

Q: What is your favorite flavor of kombucha? 

GT’s Trilogy and Divine Grape flavors were my early mainstays. Locally, Primal Delights’ Habanero Pineapple is lovely.

Q: What is your former/current other life or career?

Out of college I worked for MICROS Systems, Inc, and then Oracle Hospitality, in the IT sector. Happy to say I’m full time Kombucha Swiss Army Knife as of September.

Q: What is a saying that you live by?

I’m going to cheat on this one a bit and share something I posted ages ago on the ol’ Facebook that continues to echo for me:
With our humanity comes a responsibility to do more than just consume that which is tangible and that which is not.

The 12 virtues of a healthy human as once told by a 24 year old man-child, in no particular order:

Honesty
Love
Forgiveness
Honor
Ambition
Humility
Creativity
Passion
Moderation
Curiosity
Spontaneity
Acceptance

WEBINAR SERIES: FERMENTATION MICROSCOPY: TIPS AND TRICKS FOR THE KOMBUCHA BREWER

FERMENTATION MICROSCOPY: TIPS AND TRICKS FOR THE KOMBUCHA BREWER DATE: 7/25/18 1 pm on Zoom WHO: Keisha Harrison & Dr. Chris Curtin COST: Free for DNA/Analyte study participants, $20 Kbi Members, $40 KBI Non-Members WEBINAR SUMMARY Explore the microscopic world at work when sweetened tea becomes kombucha. During this webinar session, members of KBI will […]


Tuesday, 10th July 2018

FERMENTATION MICROSCOPY: TIPS AND TRICKS FOR THE KOMBUCHA BREWER
DATE: 7/25/18 1 pm on Zoom
WHO: Keisha Harrison & Dr. Chris Curtin
COST: Free for DNA/Analyte study participants, $20 Kbi Members, $40 KBI Non-Members

WEBINAR SUMMARY

Explore the microscopic world at work when sweetened tea becomes kombucha. During this webinar session, members of KBI will receive information on the biochemistry and microbiology of standard kombucha fermentation. Webinar topics include yeast and bacteria strains, fermentation kinetics, the kombucha microbiome, chemical composition, and microbiology techniques. Learn more about how to apply laboratory techniques such as plating of cultures, isolation of microorganisms, and PCR. Enrollment is FREE for KBI members currently participating in the Oregon State University Kombucha Genetics and Analyte study.

WEBINAR TAKEAWAYS

– The general biochemistry of mixed culture fermentations

– The role of bacteria and yeast in Kombucha production

– Laboratory techniques that can be applied to Kombucha fermentation

ABOUT THE WEBINAR INSTRUCTORS

Keisha Harrison, M.S., is a PhD candidate of Fermentation Science in the Food Science & Technology Department at Oregon State University (OSU). She received a Bachelor of Arts in Cell Biology and Biochemistry from Rice University and a Master of Science in Nutrition from the University of Houston. Keisha was drawn to Kombucha research because it is a beautifully complex system to study microbial interactions. She aims to understand the links between the microbial landscape of the Kombucha SCOBY and the sensory experience. She believes to get at the heart of Kombucha, we have to get better acquainted with it first!

 

 

 

Chris Curtin, Ph.D. is an Assistant Professor in the Food Science & Technology Department at Oregon State University (OSU). Prior to joining OSU in 2016 he lead the Biosciences research group at the Australian Wine Research Institute, where he was responsible for development of yeast strains and development of strategies to mitigate spoilage. The Curtin lab seeks to understand the role of yeasts and bacteria in production of fermented beverages, where often the same mix of species give us an array of possible outcomes. The Kombucha SCOBY embodies this complexity and has become a major focus of our research.

 

Draft Kombucha Spec Sheet

What is Kombucha? Kombucha is fermented tea! Just like yogurt is fermented milk and sauerkraut is fermented cabbage, Though the Kombucha commercial industry is new, the drink is much older as it has been consumed for hundreds of years in every country on the planet. Kombucha Ingredients Tea [Camelia sinensis], Sugar [sucrose], SCOBY [fermentation culture], […]


Friday, 29th June 2018

What is Kombucha?

Kombucha is fermented tea! Just like yogurt is fermented milk and sauerkraut is fermented cabbage, Though the Kombucha commercial industry is new, the drink is much older as it has been consumed for hundreds of years in every country on the planet.

Kombucha Ingredients

Tea [Camelia sinensis], Sugar [sucrose], SCOBY [fermentation culture], flavorings [juice, fruit, herbs, etc]

Kombucha Chemical Characteristics

pH = 2.5-3.8

Acetic acid ferment: ~1%

By comparison, vinegar is diluted to 4-8% acetic acid

ABV = 0-2% for traditional, non-alcoholic ferments

ABV = 4-8% for Kombucha beer or high alcohol Kombucha hybrid ferments

Materials Acceptable for Use with Kombucha

Stainless Steel 304 & 316, 1

Neoprene Vinyl

1 British Stainless Steel Association – 304 types are used for most applications, including handling and storage.

Draft Kombucha Components

In a draft Kombucha set up, here are the components that come into contact with product.

  • Keg Coupler – Probe – 304 Stainless Steel
  • Product Washer, between the metal contacts of Tailpiece and Coupler – Neoprene
  • Product line Tailpiece for Coupler connection – 304 Stainless Steel
  • Product line – Vinyl
  • Tower Shank – 304 Stainless Steel
  • Faucet – 304 Stainless Steel

Antimicrobial Properties of Kombucha

Kombucha is naturally antimicrobial. It’s low pH and mix of organic acids have been shown to kill known pathogens on contact.

The antimicrobial activity of Kombucha was investigated against a number of pathogenic microorganisms. According to the literature on Kombucha, acetic acid is considered to    be responsible for the inhibitory effect toward a number of microbes tested, and this is also valid in the present study. However, in this study, Kombucha proved to exert antimicrobial activities against E. coli, Sh. sonnei, Sal. typhimurium, Sal. enteritidis, and Cm. jejuni, even at neutral pH and after thermal denaturation. This finding suggests the presence of antimicrobial compounds other than acetic acid and large proteins in Kombucha. 2, 3, 4

Pathogens Sensitive to Kombucha

  • Staphylococcus aureus
  • Shigella sonnei
  • Escherichia coli
  • Aeromonashydrophila
  • Yersinia enterolitica
  • Pseudomonas aeruginosa
  • Enterobactercloacae
  • Staphylococcus epidermis
  • Campylobacter jejuni
  • Salmonella enteritidis
  • Salmonella typhimurium
  • Bacillus cereus
  • Listeria monocytogenes
  • Helicobacter pylori

For more information, check out our Best Practices Page

2 Sreeramulu, Guttapadu, Yang Zhu, and Wieger Knol. “Kombucha fermentation and its antimicrobial activity.” Journal of agricultural and food chemistry 48.6 (2000): 2589-2594.

3 Sreeramulu, G., Y. Zhu, and W. Knol. “Characterization of antimicrobial activity in Kombucha fermentation.” Acta Biotechnologica 21.1 (2001): 49-56.

4 Cetojevic-Simin, D. D., et al. “Antiproliferative and antimicrobial activity of traditional Kombucha and Satureja montana L. Kombucha.” J BUON 13.3 (2008): 395-401.

Meet Our Members – Viva Kombuxa (Mexico)

  Karla Eugenia Aldape, Viva Kombuxa, San Pedro, NL, Mexico   Q: When did you first discover kombucha? I discovered kombucha when I moved to California the year 2015. Q: Why did you start your own kombucha company? I loved kombucha and how it made me feel especially how it helped my digestion, when I moved to […]


Friday, 15th June 2018

 

Karla Eugenia Aldape, Viva Kombuxa, San Pedro, NL, Mexico

 

Q: When did you first discover kombucha?

I discovered kombucha when I moved to California the year 2015.

Q: Why did you start your own kombucha company?

I loved kombucha and how it made me feel especially how it helped my digestion, when I moved to Mexico I could not find a kombucha brand that I loved or would benefit my health so I decided to brew my own kombucha and help Mexican people feel as good as I do! Create a national brand that inspires and helps people.  

Q: What is your favorite flavor of kombucha? 

Ginger Lemon 

Q: What is your former/current other life or career?

Founder an active-wear (athleisure) brand for Women sold in Mexico 

Q: What is a saying that you live by?

See the things that you want as already yours. Know that they will come to you at need. Then let them come. Don’t fret and worry about them. Don’t think about your lack of them. Think of them as yours, as belonging to you, as already in your possession. Believe in the law of attraction.

 

Meet Our Members – InJoyCHA (CO)

  Manda Pendleton, InJoyCHA, Denver, CO, USA Q: When did you first discover kombucha? I first discovered kombucha in 2008. A friend was brewing it and gave me a sip and my first reaction was ‘this tastes like vinegar’! I am partial to vinegar and also creating healing herbal products so she gave me my […]


Friday, 1st June 2018

 

Manda Pendleton, InJoyCHA, Denver, CO, USA

Q: When did you first discover kombucha?

I first discovered kombucha in 2008. A friend was brewing it and gave me a sip and my first reaction was ‘this tastes like vinegar’! I am partial to vinegar and also creating healing herbal products so she gave me my first scoby and an obsession was born!  When I tried my first herbal kombucha I thought ‘this doesn’t taste like those herbs’ so I decided to combine my skill of mixology and herbal formulation with fermenting probiotic beverages.  

Q: Why did you start your own kombucha company?

After home-brewing for several years I got such a great response from friends and whomever I sampled to that I decided to start my company in 2011, however I did not have the proper funding to make it feasible at that point, so I closed my business and then launched it again April 22nd 2013. I market 12 unique herbal flavors of my product @InJoyCHA with the energetic aspects of gem/flower essences and reiki universal energy. 

Q: What is your favorite flavor of kombucha? 

Whenever I see a new product on the market I always give it a try. Hence I have quite a collection of bottles in my marketing research.  I am partial to small batch products not brewed in metal and love the energy of passiflora incarnata so I would have to say Rainbow Cloud kombucha passion fruit brewed in oak barrels out of Shelton, WA. I love their positive message and sacred geometry/hieroglyphics on their label.

Q: What is your former/current other life or career?

I am a Clinical Herbalist and Nutritionist by trade with 20 years of herbal formulating under my belt. I also do private health consultations and consult businesses for herbal and nutritional formulating. I have been educating the community locally/internationally regarding  herbal medicine, natural health, Yoga and fermentation for over 15 years and this is a true Joy for me to share this important knowledge. I have a growing line of products besides InJoyCHA including Xun, Tibicos, herbal tonics, teas, topical balms and skincare as well as sauerkraut, pickles, and other probiotic foods. 

Q: What is a saying that you live by?

May you realize your innate power to heal and transform yourself! U R love U R light

 

 

Meet Our Members – Moss Beach Kombucha (CA)

Douglas Nelson, Moss Beach Kombucha, Moss Beach, CA, USA   Q: When did you first discover kombucha? ​ A friend in Oakland turned me on to making it in about 2009 and I’ve been making it ever since.​   Q: Why did you start your own Kombucha company?   ​I’ve been looking for my next […]


Tuesday, 29th May 2018

Douglas Nelson, Moss Beach Kombucha, Moss Beach, CA, USA

 

Q: When did you first discover kombucha?

​ A friend in Oakland turned me on to making it in about 2009 and I’ve been making it ever since.​
 
Q: Why did you start your own Kombucha company?
 
​I’ve been looking for my next challenge. I knew my KT was good, but I hadn’t had any commercial KT for years. I tried what was out there and I figure mine is in league with what is being sold.​

 

Q: What is your favorite flavor of kombucha? 

​I like House Citrus Hibiscus and Marin Kombucha’s Pinot Sage.​

 

Q: What is your former/current other life or career?

​I did some acting in NYC, I’ve produced a few CD’s (you can hear my stuff at ​goo.gl/5Z65gO)​, and I’ve been a data systems developer in several industries, including finance, energy efficiency, and CRM. My latest project was finishing culinary school in San Francisco last year. Time to put that to use.

 

Q: What is a saying that you live by?

​There is a crack in everything. That’s how the light gets in​.

Kombucha Chemistry: What’s in a brew?

by Keisha Rose Harrison, MS (PhD Candidate at Oregon State University) Kombucha diversification is on the rise. As more brewers rush to the scene, new and old producers alike have to answer the question: what defines our Kombucha? The uniqueness of a kombucha product can be divided into two descriptors: the microbial community and the […]


Tuesday, 22nd May 2018

by Keisha Rose Harrison, MS (PhD Candidate at Oregon State University)

Kombucha diversification is on the rise. As more brewers rush to the scene, new and old producers alike have to answer the question: what defines our Kombucha? The uniqueness of a kombucha product can be divided into two descriptors: the microbial community and the chemical composition. The flavor, the aroma, and the mouthfeel are all sensory attributes that can be related back to the chemical composition. Additionally, the fermentation of sweetened tea can produce compounds (i.e. organic acids and polyphenols) associated with reports of health benefits. Kombucha reportedly may consist of organic acids, ethanol, vitamins, polyphenols, catechins, amino acids, and antibiotics. The measure of these bioactive compounds is paramount in determining the consumer’s experience.

The symbiotic microbes within the Kombucha culture work in tandem to catabolize the sweetness from the starter material. Yeast possess an enzyme invertase which breaks down sucrose (cane sugar) into simpler hexose sugars. Bacteria and a few types of yeast can further oxidize glucose and fructose into organic acids, including acetic acid. The extent to which these molecules are broken down by the microbes determines the resulting chemical composition. Factors that influence these reactions include: microbial population, sugar concentration, fermentation temperature, and fermentation duration. A table of varying fermentation conditions and common compound concentrations can be found below.

Ethanol and pH have become standard measurements for tracking the proper course of Kombucha fermentations. Although pH is effective in clarifying the endpoint, it fails to discern the various organic acid components. Some of the more commonly reported organic acids include, acetic acid, gluconic acid, glucuronic, lactic acid, citric acid, and formic acid. Acetic acid, typically the dominant acid, is produced when acetic acid bacteria oxidize ethanol produced by sacchrolytic yeast. It is detectable at 175mg/L and carries a distinguishable pungent sourness noticeable in flavor and aroma. Fruit notes can characteristic such as apple, black current, and pineapple. Meanwhile, lactic acid is produced when fructophilic lactic acid bacteria (LAB) anaerobically ferment carbohydrates. It has very little to no aroma and contributes a “tang” sour character, distinguishable at 400 mg/L. The less abundant citric acid gives of a “tart” sour taste at a threshold of 60 mg/L. The balance of these flavor-active organic acids determines how your Kombucha tastes and smells!

Increasing focus has been directed towards glucoronic acid (GlcUA) because of its association with health benefits. GlcUA acid is formed during glucose oxidation. According to one study by Nguyen (2015), the bacteria Gluconoacetobacter intermedius is capable of producing detectable levels of GlcUA acid which varies in response to yeast abundance. What’s so special about this organic acid? Numerous studies have found GlcUA to confer detoxifying benefits by binding to xenobiotics (toxins) in the liver and making them easier to eliminate. The amount of GlcUA produced during Kombucha fermentation depends substantially on fermentation temperature and the microbial composition. In addition to GlcUA, other health-related compounds, i.e. vitamin C, folic acid, polyphenols, and catechins, have been found in Kombucha, making it a likely treasure trove of beneficial compounds!

How can you profile your brew’s unique chemical composition? KBI is partnering with Oregon State University to conduct a Kombucha Analyte Study. By participating, you will submit a sample of finished product to be run on Nuclear Magnetic Resonance equipment to detect residual sugars, organic acids, caffeine, and additional metabolites with high fidelity. To learn more about participation, visit: https://kombuchabrewers.org/kbi-osu-scoby-genomics-analyte-study/.

References:

Chen, C., & Liu, B. Y. (2000). Changes in major components of tea fungus metabolites during prolonged fermentation. Journal of Applied Microbiology, 89(5), 834-839.

De Filippis, F., Troise, A. D., Vitaglione, P., & Ercolini, D. (2018). Different temperatures select distinctive acetic acid bacteria species and promotes organic acids production during Kombucha tea fermentation. Food Microbiology.

Jayabalan, R., Marimuthu, S., & Swaminathan, K. (2007). Changes in content of organic acids and tea polyphenols during kombucha tea fermentation. Food Chemistry, 102(1), 392-398.

Jayabalan, R., Malbaša, R. V., Lončar, E. S., Vitas, J. S., & Sathishkumar, M. (2014). A review on kombucha tea—microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4), 538-550.

Lončar, E. S., Petrovič, S. E., Malbača, R. V., & Verac, R. M. (2000). Biosynthesis of glucuronic acid by means of tea fungus. Food/Nahrung, 44(2), 138-139.

Nguyen, N. K., Nguyen, P. B., Nguyen, H. T., & Le, P. H. (2015). Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid. LWT-Food Science and Technology, 64(2), 1149-1155.

Sievers, M., Lanini, C., Weber, A., Schuler-Schmid, U., & Teuber, M. (1995). Microbiology and fermentation balance in a kombucha beverage obtained from a tea fungus fermentation. Systematic and Applied Microbiology, 18(4), 590-594.

Sparrow, J. (2015) Wild Brews, Brewer’s Publications

Velićanski, A., Cvetković, D,& Markov, S. (2013). Characteristics of Kombucha fermentation on medicinal herbs from Lamiaceae family. Romanian Biotechnological Letters, 18(1), 8034-8042.

Talawat, S., Ahantharik, P., Laohawiwattanakul, S., Premsuk, A., & Ratanapo, S. (2006). Efficacy of fermented teas in antibacterial

Meet Our Members – A Lively Brew (AR)

  Will and Jenny Lively, A Lively Brew, Hot Springs, AR, USA   Q: When did you first discover kombucha?  My husband and I were living in Austin when we first stumbled across kombucha. With so many great brands with such a wide range of flavors and styles, we were hooked.  Q: Why did you […]


Saturday, 12th May 2018

 

Will and Jenny Lively, A Lively Brew, Hot Springs, AR, USA

 

Q: When did you first discover kombucha?

 My husband and I were living in Austin when we first stumbled across kombucha. With so many great brands with such a wide range of flavors and styles, we were hooked. 

Q: Why did you start your own kombucha company?

We first started in 2012 in Austin, until we relocated to current home base, Arkansas in 2013.

Q: What is your favorite flavor of kombucha? 

Wunder-Pilz from Austin has always been near and dear to us.

Q: What is your former/current other life or career?

My husband and I both went to culinary school and have been known to moonlight a catering event or two. 

Q: What is a saying that you live by?

“A man is a success if he gets up in the morning and goes to bed at night and in between does what he wants to do.” -Bob Dylan

 

The Kombucha SCOBY: A Symbiotic Culture Of What Exactly?

By Keisha Rose Harrison, MS (PhD Candidate at Oregon State University) Kombucha, a funky fermented tea, is a time immemorial beverage that has made appearances across the globe in various forms for eons. This beverage of unknown origin is shrouded in many mysteries. The potential health benefits, fermentation kinetics, and alcohol production are a few […]


Thursday, 10th May 2018
By Keisha Rose Harrison, MS (PhD Candidate at Oregon State University)
FERMENTATION

Kombucha, a funky fermented tea, is a time immemorial beverage that has made appearances across the globe in various forms for eons. This beverage of unknown origin is shrouded in many mysteries. The potential health benefits, fermentation kinetics, and alcohol production are a few aspects that continue to baffle kombucha lovers and producers alike. Meanwhile, one area where fermentation researchers are making ground is in defining the microbiology of the Kombucha SCOBY.

In comparison to other fermented beverages, i.e. wine and beer, the amount of research done on the Kombucha SCOBY is sparse. It was known in even the earliest studies, from back in the 1960s that the starter colony was made up of both yeast and bacteria. However, there was little known about which organisms drove the fermentation, contributed to flavor, and fought off spoilage. Until recently, scientists relied upon culture-based methods (isolating microbes on media plates) to identify yeast and bacteria organisms. This technique, although cost-effective and accessible, is prone to error. Despite its limitations, scientists (Teoh et al, 2004; Liu et al, 1996), were able to come to consensus about the role of acetic acid bacteria (AAB) and saccharolytic (“sugar eating”) yeast.

By the early 2000s, we understood that AAB converted ethanol to acetic acid and built the SCOBY. Acetobacter and Gluconoacterobacter, genera of AAB, were claimed as staples and have since been renamed to fit under the genus Komagataeibacter. There was less agreement about which yeast cells eat cane sugar to produce simple sugar hexoses for the bacteria. There are accounts of Zygosaccharomyces, Schizosaccharomyces, Brettanomyces, and Candida. However, Teoh (2004) warns us that identifying organisms based upon isolation methods is generally unsuccessful.

The DNA sequencing boom changed the name of the game! With the advent of affordable and reliable genetic sequencing, high-resolution taxonomic identification became a thing of reality. First scientists used sequencing technology to characterize isolated organism as a more robust culture dependent approach. However, with next generation sequencing (NGS), we could finally for the first time identify both culturable and nonculturable organisms. With NGS technology, scientists confirmed Komagatebacter as the dominant Kombucha bacteria (Marsh, 2014; Chakravorty, 2016) revealed that lactic acid and thermophilic bacteria that are likely dependent upon Kombucha preparation and region. (See table below for comprehensive list of evaluated microorganisms) These studies finally give us an in-depth analysis of kombucha microflora.

What does this mean to brewers? The recent application of advanced sequencing proved that we can apply high-fidelity identification tools to kombucha. With the precedent already set, we can now focus on designing experiments that address the larger questions. For instance, “what is common to commercial SCOBY?” and “which organisms are found in kombucha with high organic acid production?” By sequencing a mass number of SCOBY from around the globe, we will have the potential to link microbes with the chemical composition of finished kombucha products. Additionally, we will get closer to curating reproducibility and troubleshooting problematic batches.

How you can get involved? KBI members have the opportunity to participate in highly discounted genetic sequencing ($125 per sample) of the Kombucha SCOBY and broth. Fermentation scientists at Oregon State University and creating a database of microbial populations from commercial kombucha. Not only will you have the opportunity to see what your SCOBY is comprised of, but you will also be able to see your powerhouse compares to the average SCOBY. This is just the first step in understanding how the microbial population relates to kombucha features.

For more information in registering, visit: https://kombuchabrewers.org/kbi-osu-scoby-genomics-analyte-study/

Referenced Papers

  • Coton, Monika, et al. “Unraveling Microbial Ecology of Industrial-Scale Kombucha Fermentations by Metabarcoding and Culture-Based Methods.” FEMS Microbiology Ecology, vol. 93, no. 5, 2017, pp. FEMS Microbiology Ecology, 2017, Vol. 93(5).
  • Chakravorty, Somnath, et al. “Kombucha tea fermentation: Microbial and biochemical dynamics.” International journal of food microbiology 220 (2016): 63-72.
  • El-Salam, S. S. A. (2012). 16S rRNA gene sequence detection of acetic acid bacteria isolated from tea kombucha. New York Science Journal, 5(3), 55-61.
  • Jankovic, I., Stojanovic, M., 1994. Microbial and chemical composition, growth, therapeutical and antimicrobial characteristics of tea fungus. Mikrobiologija 33, 25 – 34.
  • Liu, C-H., et al. “The isolation and identification of microbes from a fermented tea beverage, Haipao, and their interactions during Haipao fermentation.” Food Microbiology 13.6 (1996): 407-415.
  • Marsh, Alan J., et al. “Sequence-based analysis of the bacterial and fungal compositions of multiple kombucha (tea fungus) samples.” Food microbiology 38 (2014): 171-178.
  • Mayser, P., Gromme, S., Leitzmann, C., Gru¨nder, K., 1995. The yeast spectrum of the ‘tea fungus Kombucha’. Mycoses 38, 289 – 295
  • Shade, Ashley, D. H. Buckley, and S. H. Zinder. “The kombucha biofilm: a model system for microbial ecology.” Final report on research conducted during the Microbial Diversity course. Marine Biological Laboratories, Woods Hole, MA (2011).
  • Teoh, Ai Leng, Gillian Heard, and Julian Cox. “Yeast ecology of Kombucha fermentation.” International journal of food microbiology 95.2 (2004): 119-126.

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  • Meet Our Members – Pigeon Cove Ferments (MA)
  • Meet Our Members – Tonica Kombucha (CAN)

Contact Us

8950 W Olympic Blvd, Ste 494,
Beverly Hills, CA 90211

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